5F0O
Cohesin subunit Pds5 in complex with Scc1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97942 |
| Spacegroup name | H 3 |
| Unit cell lengths | 235.400, 235.400, 94.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.486 - 3.500 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.29070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.837 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.490 |
| High resolution limit [Å] | 3.500 |
| Rmerge | 0.079 |
| Number of reflections | 22568 |
| <I/σ(I)> | 11.2 |
| Completeness [%] | 99.9 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 50 mM sodium cacodylate pH 6.5, 1.4 - 1.6 M ammonium sulphate and 5 mM magnesium acetate |
