5F0G
Structure of the glutathione transferase delta 2 from Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.500, 87.500, 53.200 |
| Unit cell angles | 90.00, 113.90, 90.00 |
Refinement procedure
| Resolution | 19.950 - 1.600 |
| R-factor | 0.1739 |
| Rwork | 0.172 |
| R-free | 0.21710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ein |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.274 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | |
| Number of reflections | 51278 | |
| <I/σ(I)> | 15.09 | 2.03 |
| Completeness [%] | 97.6 | 97 |
| Redundancy | 3.38 | 3.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.1 M succinic acid, phosphate, glycin (SPG buffer), 25% PEG 1500, pH = 4 |
