5F06
Crystal structure of glutathione transferase F7 from Populus trichocarpa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979769 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.104, 90.365, 97.149 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.420 - 1.800 |
| R-factor | 0.1807 |
| Rwork | 0.179 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ri6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.861 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.570 | 1.820 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.103 | 0.664 |
| Number of reflections | 50544 | |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 277 | 16% PEG 4000, 10% 2-propanol, Na Hepes pH 7.5, 0.2 AS |
