5EZL

Crystal Structure of Fab of parasite invasion inhibitory antibody c1 - monoclinic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-06
Detector	PSI PILATUS 6M
Wavelength(s)	1.000020
Spacegroup name	C 1 2 1
Unit cell lengths	101.420, 108.900, 97.400
Unit cell angles	90.00, 97.75, 90.00

Refinement procedure

Resolution	48.200 - 2.430
R-factor	0.1898
Rwork	0.187
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ezi 5ezj
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.200		2.490
High resolution limit [Å]	2.430	10.870	2.430
Rmerge	0.148	0.025	1.411
Rmeas	0.176	0.030	1.666
Total number of observations	133813
Number of reflections	39274	455	2911
<I/σ(I)>	7.77	31.45	0.99
Completeness [%]	99.3	97.2	99.6
Redundancy	3.4		3.5
CC(1/2)	0.992	0.999	0.313

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.1 M HEPES-NaOH pH 7.0, 20% PEG 8000