5EZB
Chicken prestin STAS domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 81.551, 56.919, 101.140 |
| Unit cell angles | 90.00, 96.19, 90.00 |
Refinement procedure
| Resolution | 46.585 - 2.300 |
| R-factor | 0.2217 |
| Rwork | 0.220 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3llo |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.295 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.590 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.069 | 0.665 |
| Number of reflections | 19970 | |
| <I/σ(I)> | 18.9 | 2.8 |
| Completeness [%] | 96.1 | 96.9 |
| Redundancy | 6.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris pH 8.5, 1.5 M Ammonium Sulphate, 12% (v/v) glycerol, 0.3 M Ammonium oxalate |
