5EZA
A de novo designed heptameric coiled coil CC-Hept-I18C-L22H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2014-04-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 62.240, 61.370, 69.415 |
| Unit cell angles | 90.00, 109.98, 90.00 |
Refinement procedure
| Resolution | 53.590 - 1.760 |
| R-factor | 0.1684 |
| Rwork | 0.166 |
| R-free | 0.20660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pna |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.856 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.590 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.097 | 0.396 |
| Number of reflections | 24225 | |
| <I/σ(I)> | 7.6 | 2.6 |
| Completeness [%] | 99.1 | 98.3 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.1 M MgSO4, 0.1 M HEPES at pH 7.0 with 15% w/v PEG 4,000 |
