5EZ2

Sandercyanin Fluorescent Protein (SFP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-10
Detector	MAR CCD 130 mm
Wavelength(s)	0.987
Spacegroup name	P 63 2 2
Unit cell lengths	159.266, 159.266, 84.157
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.990 - 1.849
R-factor	0.1912
Rwork	0.190
R-free	0.21250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f6z
RMSD bond length	0.007
RMSD bond angle	1.735
Data reduction software	iMOSFLM
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.990	1.915
High resolution limit [Å]	1.849	1.849
Number of reflections	53181
<I/σ(I)>	15.48
Completeness [%]	98.5
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	0.1M Bis-Tris, 1M NaCl, PEG 400