5EY9
Structure of FadD32 from Mycobacterium marinum complexed to AMPC12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.790, 100.150, 212.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.700 - 2.500 |
| R-factor | 0.1875 |
| Rwork | 0.185 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kxw |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.786 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.484 | |
| Number of reflections | 53766 | |
| <I/σ(I)> | 7.7 | 2.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.7 | 285 | PEG 6000 28% TrisHCl 100 mM |
