5EY8
Structure of FadD32 from Mycobacterium smegmatis complexed to AMPC20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 4 |
| Unit cell lengths | 164.040, 164.040, 231.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.442 - 3.500 |
| R-factor | 0.2259 |
| Rwork | 0.223 |
| R-free | 0.28620 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.011 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.200 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 72026 | |
| <I/σ(I)> | 4.6 | 1.6 |
| Completeness [%] | 93.9 | 95.7 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | PEG 1000 TrisHCl 100 mM, pH 8.2 - 8.7 |
