5EXY
Crystal structure of in cellulo recombinant CPV1 Polyhedra
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 103.180, 103.180, 103.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.320 - 1.550 |
| R-factor | 0.1321 |
| Rwork | 0.128 |
| R-free | 0.16690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oh5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BUSTER (2.10) |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
| Rmerge | 0.249 | 0.096 | 0.937 |
| Rmeas | 0.257 | 0.105 | |
| Rpim | 0.111 | 0.042 | 0.466 |
| Total number of observations | 155448 | ||
| Number of reflections | 26475 | ||
| <I/σ(I)> | 4.3 | ||
| Completeness [%] | 99.6 | 99 | 99.4 |
| Redundancy | 5.9 | 6 | 4.8 |
| CC(1/2) | 0.990 | 0.519 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 300 | in vivo crystallization in the cytoplasm of the cell |
