5EXX
AAA+ ATPase FleQ from Pseudomonas aeruginosa bound to c-di-GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 137.836, 137.836, 172.773 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.654 - 3.311 |
| R-factor | 0.2644 |
| Rwork | 0.263 |
| R-free | 0.28970 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.536 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.600 | 3.600 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.085 | 0.715 |
| Number of reflections | 13582 | |
| <I/σ(I)> | 17.9 | 1.8 |
| Completeness [%] | 88.5 | 47.8 |
| Redundancy | 5.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.05 M Bis-Tris pH 6.5, 30% pentaerythritol ethoxylate (15/4 EO/OH) and 0.05 M ammonium sulfate |
