5EXX

AAA+ ATPase FleQ from Pseudomonas aeruginosa bound to c-di-GMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-12
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9792
Spacegroup name	P 63 2 2
Unit cell lengths	137.836, 137.836, 172.773
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.654 - 3.311
R-factor	0.2644
Rwork	0.263
R-free	0.28970
RMSD bond length	0.013
RMSD bond angle	2.536
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.600	3.600
High resolution limit [Å]	3.300	3.300
Rmerge	0.085	0.715
Number of reflections	13582
<I/σ(I)>	17.9	1.8
Completeness [%]	88.5	47.8
Redundancy	5.4	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.05 M Bis-Tris pH 6.5, 30% pentaerythritol ethoxylate (15/4 EO/OH) and 0.05 M ammonium sulfate