5EXV
Crystal structure of heme binding protein HutX from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.200, 80.880, 111.090 |
| Unit cell angles | 90.00, 95.55, 90.00 |
Refinement procedure
| Resolution | 48.657 - 2.901 |
| R-factor | 0.248 |
| Rwork | 0.246 |
| R-free | 0.27790 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.316 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.660 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.535 | |
| Number of reflections | 23690 | |
| <I/σ(I)> | 10.3 | 2.34 |
| Completeness [%] | 98.3 | 97 |
| Redundancy | 3.41 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | HEPES, PEG400, ammonium sulfate |
