5EXP
AAA+ domain of FleQ from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-12 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.4592 |
| Spacegroup name | P 65 |
| Unit cell lengths | 104.907, 104.907, 42.752 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.400 - 1.800 |
| R-factor | 0.1702 |
| Rwork | 0.168 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.004 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.400 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.138 | 0.970 |
| Number of reflections | 25124 | |
| <I/σ(I)> | 14.7 | 2.6 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 10.4 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-Tris pH 6.5, 0.2 M ammonium sulfate and 25% w/v PEG3350 |
