5EXH
Crystal structure of mTET3-CXXC domain in complex with 5-carboxylcytosine DNA at 1.3 Angstroms resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.977 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.959, 27.230, 47.060 |
Unit cell angles | 90.00, 104.44, 90.00 |
Refinement procedure
Resolution | 32.633 - 1.300 |
R-factor | 0.169 |
Rwork | 0.168 |
R-free | 0.18740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hp1 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.523 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.061 | 0.256 |
Number of reflections | 23079 | |
<I/σ(I)> | 25 | 5.14 |
Completeness [%] | 99.7 | 100 |
Redundancy | 4.2 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Hepes, pH 7.5, 50 mM, calcium chloride, 41% PEG200 |