5EWN

Crystal structure of the human astrovirus 1 capsid protein core domain at 2.6 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	66.243, 71.098, 158.911
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.466 - 2.602
R-factor	0.1941
Rwork	0.192
R-free	0.23410
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.601
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.470	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.105
Number of reflections	23541
<I/σ(I)>	19.66	3.41
Completeness [%]	99.3	98.4
Redundancy	6.9	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	295	PEG 6000, sodium acetate, lithium chloride
1	VAPOR DIFFUSION, HANGING DROP	8.4	295	PEG 6000, sodium acetate, lithium chloride