5EWE

Ternary complex of human DNA polymerase eta inserting rCTP opposite template G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 61
Unit cell lengths	99.282, 99.282, 81.895
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.990 - 1.660
R-factor	0.1731
Rwork	0.171
R-free	0.20740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o3n
RMSD bond length	0.007
RMSD bond angle	1.007
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.690
High resolution limit [Å]	1.660	1.660
Rmerge	0.098	0.978
Number of reflections	54114
<I/σ(I)>	19.2	2.4
Completeness [%]	100.0	100
Redundancy	8.9	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	NaMES (pH 6.0), polyethylene glycol monomethyl ether 2000, and calcium chloride