5EWD
Crystal structure of the human BRPF1 bromodomain in complex with SEED18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.670, 60.670, 63.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.356 - 1.580 |
| R-factor | 0.2005 |
| Rwork | 0.199 |
| R-free | 0.22570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lc2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.666 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.360 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.029 | 0.359 |
| Number of reflections | 18839 | |
| <I/σ(I)> | 34.7 | 5.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.6 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M Sodium Acetate, pH5.5, 0.15 M Sodium Nitrate, 20% PEG3350 |
