5EVI
Crystal Structure of Beta-Lactamase/D-Alanine Carboxypeptidase from Pseudomonas syringae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97926 |
Spacegroup name | P 1 |
Unit cell lengths | 53.869, 72.645, 93.770 |
Unit cell angles | 89.94, 90.01, 90.08 |
Refinement procedure
Resolution | 36.320 - 1.800 |
R-factor | 0.16493 |
Rwork | 0.163 |
R-free | 0.20081 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.473 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.419 | |
Number of reflections | 128402 | |
<I/σ(I)> | 12.44 | 2.49 |
Completeness [%] | 97.5 | 96.2 |
Redundancy | 2.2 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 2.0 M Ammonium Sulfate 0.1 M Bis-Tris:HCl pH 6.5 |