5EVH

Crystal structure of known function protein from Kribbella flavida DSM 17836

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97926
Spacegroup name	P 41 21 2
Unit cell lengths	45.908, 45.908, 140.479
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.462 - 1.852
R-factor	0.1729
Rwork	0.172
R-free	0.19970
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.582
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.089	0.032	1.442
Rmeas	0.092	0.033	1.489
Rpim	0.022	0.008	0.368
Total number of observations	243644
Number of reflections	13604
<I/σ(I)>	8.8
Completeness [%]	99.8	97.2	100
Redundancy	17.9	15.6	15.6
CC(1/2)		0.998	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	Ammonium Actate, Sodium Acetate pH 4.6, PEG4000, glycerol