5EV0
Crystal structure of ragweed profilin Amb a 8 in complex with poly-Pro14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.132, 40.342, 60.256 |
| Unit cell angles | 90.00, 104.42, 90.00 |
Refinement procedure
| Resolution | 45.470 - 2.100 |
| R-factor | 0.1947 |
| Rwork | 0.193 |
| R-free | 0.23420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5em0 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.759 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.079 | 0.427 |
| Number of reflections | 14967 | |
| <I/σ(I)> | 22.7 | 2.7 |
| Completeness [%] | 98.4 | 92.4 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1 M HEPES, pH 6.5, 1.4 M sodium citrate |
