5EUX
Rat prestin STAS domain in complex with thiocyanate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-09 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 2.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 62.365, 62.365, 66.755 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.988 - 2.038 |
| R-factor | 0.1648 |
| Rwork | 0.163 |
| R-free | 0.19770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3llo |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.259 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.990 | 2.090 |
| High resolution limit [Å] | 2.038 | 2.040 |
| Rmerge | 0.081 | 1.138 |
| Number of reflections | 9671 | |
| <I/σ(I)> | 22.2 | 2.1 |
| Completeness [%] | 97.4 | 87.3 |
| Redundancy | 17.4 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Mes pH 6.5, 1.8 M Ammonium Sulphate, 250 mM NaSCN, 5% (w/v) PEG400, 0.1% (w/v) octyl-beta-D-glucopyranoside |
