5EUW
Rat prestin STAS domain in complex with nitrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-20 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.2 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 62.223, 62.223, 66.873 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.959 - 1.810 |
R-factor | 0.1655 |
Rwork | 0.164 |
R-free | 0.19040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3llo |
RMSD bond length | 0.008 |
RMSD bond angle | 1.057 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.960 | 1.850 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.075 | 1.143 |
Number of reflections | 14062 | |
<I/σ(I)> | 15 | 1.7 |
Completeness [%] | 99.9 | 97.2 |
Redundancy | 6.4 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Mes pH 6.5, 1.8 M Ammonium Sulphate, 100 mM NaNO3, 5% (w/v) PEG400, 0.1% (w/v) octyl-beta-D-glucopyranoside |