5EUV

Crystal structure of a cold-adapted dimeric beta-D-galactosidase from Paracoccus sp. 32d strain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.967
Spacegroup name	P 21 21 21
Unit cell lengths	78.490, 106.240, 199.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.770 - 2.400
R-factor	0.16856
Rwork	0.165
R-free	0.23088
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gm8
RMSD bond length	0.017
RMSD bond angle	1.874
Data reduction software	XDS
Data scaling software	XDS
Phasing software	BALBES
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.120	0.710
Number of reflections	65576
<I/σ(I)>	11.67	2.07
Completeness [%]	99.4	97.9
Redundancy	4.39	3.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	24% PEG MME 2000, 0.1M ammonium acetate, Bis-Tris pH 6.0