5EUU
Rat prestin STAS domain in complex with chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 2.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 62.133, 62.133, 66.587 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.067 - 1.870 |
| R-factor | 0.1593 |
| Rwork | 0.157 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3llo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.764 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.850 | 1.910 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.071 | 0.749 |
| Number of reflections | 12555 | |
| <I/σ(I)> | 26.2 | 2.2 |
| Completeness [%] | 98.8 | 84.2 |
| Redundancy | 15.6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Mes pH 6.5, 1.8 M Ammonium Sulphate, 5% (w/v) PEG400, 0.1% (w/v) octyl-beta-D-glucopyranoside. Crystals were soaked with precipitant solution supplemented with 1 M Ammonium Chloride |
