5EUT

Crystal structure of phosphatidyl inositol 4-kinase II alpha in the apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-30
Detector	PSI PILATUS 6M
Wavelength(s)	0.9796
Spacegroup name	P 21 21 2
Unit cell lengths	80.006, 101.515, 78.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.967 - 2.802
R-factor	0.2486
Rwork	0.246
R-free	0.28890
RMSD bond length	0.003
RMSD bond angle	0.905
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.970
High resolution limit [Å]	2.802
Number of reflections	16220
<I/σ(I)>	8.38
Completeness [%]	99.2
Redundancy	7.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.1 M bicine/Trizma base pH 8.5