5EUR
Hypothetical protein SF216 from shigella flexneri 5a M90T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-30 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97970 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.399, 50.411, 99.921 |
| Unit cell angles | 90.00, 104.10, 90.00 |
Refinement procedure
| Resolution | 24.478 - 1.698 |
| R-factor | 0.2062 |
| Rwork | 0.205 |
| R-free | 0.24050 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.907 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.698 | 1.700 |
| Rmerge | 0.013 | 0.322 |
| Number of reflections | 44562 | |
| <I/σ(I)> | 36.9 | 6.2 |
| Completeness [%] | 95.1 | 88.4 |
| Redundancy | 4.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium HEPES, 2 M ammonium sulfate, 2% v/v PEG 400 |
