5EUK
Cetuximab Fab in complex with F3H meditope variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-10-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.930, 82.060, 212.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.830 - 2.500 |
| R-factor | 0.1746 |
| Rwork | 0.171 |
| R-free | 0.23370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gw1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.193 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_1426) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.830 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.126 | 0.775 |
| Number of reflections | 39428 | |
| <I/σ(I)> | 11.3 | 1.8 |
| Completeness [%] | 99.2 | 92.9 |
| Redundancy | 3.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | 0.1 M citric acid, 0.1 M sodium phosphate dibasic, 0.4 M potassium phosphate dibasic, 1.6 M sodium phosphate monobasic |
