5EUK

Cetuximab Fab in complex with F3H meditope variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-10-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	63.930, 82.060, 212.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.830 - 2.500
R-factor	0.1746
Rwork	0.171
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gw1
RMSD bond length	0.008
RMSD bond angle	1.193
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (dev_1426)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.830	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.126	0.775
Number of reflections	39428
<I/σ(I)>	11.3	1.8
Completeness [%]	99.2	92.9
Redundancy	3.8	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	293	0.1 M citric acid, 0.1 M sodium phosphate dibasic, 0.4 M potassium phosphate dibasic, 1.6 M sodium phosphate monobasic