5ETA
Structure of MAPK14 with bound the KIM domain of the Toxoplasma protein GRA24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.091, 61.900, 75.671 |
| Unit cell angles | 105.62, 96.31, 114.65 |
Refinement procedure
| Resolution | 40.132 - 2.800 |
| R-factor | 0.2226 |
| Rwork | 0.219 |
| R-free | 0.29030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.962 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.790 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.690 | |
| Number of reflections | 43826 | |
| <I/σ(I)> | 8.3 | 1.7 |
| Completeness [%] | 96.6 | 96.8 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M MgCl2, 0.1M Tris/HCl pH 8.5, 25-28% PEG 3350 |
