5ETA
Structure of MAPK14 with bound the KIM domain of the Toxoplasma protein GRA24
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.873 |
Spacegroup name | P 1 |
Unit cell lengths | 52.091, 61.900, 75.671 |
Unit cell angles | 105.62, 96.31, 114.65 |
Refinement procedure
Resolution | 40.132 - 2.800 |
R-factor | 0.2226 |
Rwork | 0.219 |
R-free | 0.29030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.962 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.790 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.690 | |
Number of reflections | 43826 | |
<I/σ(I)> | 8.3 | 1.7 |
Completeness [%] | 96.6 | 96.8 |
Redundancy | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M MgCl2, 0.1M Tris/HCl pH 8.5, 25-28% PEG 3350 |