5ET5

Human muscle fructose-1,6-bisphosphatase in active R-state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-22
Detector	RAYONIX MX-225
Wavelength(s)	0.9184
Spacegroup name	I 41 2 2
Unit cell lengths	72.564, 72.564, 235.348
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.280 - 1.670
R-factor	0.194
Rwork	0.193
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ifa
RMSD bond length	0.019
RMSD bond angle	1.791
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.280	1.770
High resolution limit [Å]	1.670	1.670
Rmerge	0.052	0.900
Number of reflections	37062
<I/σ(I)>	29.89	3.08
Completeness [%]	99.8	99.4
Redundancy	13.03	13.18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	292	10mM magnesium chloride, 2M sodium chloride, 10%PEG6000, 10mM Tris