5ES7
Crystal structure of the F-A domains of the LgrA initiation module soaked with FON, AMPcPP, and valine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2014-11-27 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 160.943, 160.943, 137.581 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.982 - 2.805 |
| R-factor | 0.2273 |
| Rwork | 0.226 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5es6 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.677 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
| Rmerge | 0.082 | 0.034 | |
| Rmeas | 0.085 | 0.036 | |
| Rpim | 0.024 | 0.010 | 0.493 |
| Total number of observations | 594075 | ||
| Number of reflections | 42877 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 95.8 | 99.9 | 78.6 |
| Redundancy | 13.9 | 13.5 | 11.3 |
| CC(1/2) | 0.999 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2 M Na-formate, 0.1 M Na-Acetate pH 5.3 |
