5ES6
Crystal structure of the first two domains of the initiation module of LgrA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2014-09-25 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 161.320, 161.320, 138.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.954 - 2.460 |
R-factor | 0.2239 |
Rwork | 0.223 |
R-free | 0.25070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1amu |
RMSD bond length | 0.002 |
RMSD bond angle | 0.630 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 63.945 | 63.945 | 2.590 |
High resolution limit [Å] | 2.460 | 7.780 | 2.460 |
Rmerge | 0.044 | 1.289 | |
Rmeas | 0.107 | ||
Rpim | 0.034 | 0.016 | 0.444 |
Total number of observations | 649606 | 20880 | 88471 |
Number of reflections | 66592 | ||
<I/σ(I)> | 12.5 | 30.6 | 1.7 |
Completeness [%] | 100.0 | 99.7 | 100 |
Redundancy | 9.8 | 8.9 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 2 M Na-formate, 0.1 M Na-Acetate pH 5.3 |