5ES2
The crystal structure of a functionally uncharacterized protein LPG0634 from Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.070, 97.322, 100.447 |
| Unit cell angles | 90.00, 96.08, 90.00 |
Refinement procedure
| Resolution | 36.202 - 2.600 |
| R-factor | 0.1942 |
| Rwork | 0.191 |
| R-free | 0.25320 |
| Structure solution method | SAD |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.342 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.106 | 0.701 |
| Number of reflections | 35558 | |
| <I/σ(I)> | 24.7 | 3.06 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.2M LiSO4, 0.1M HEPES, 25% PEG335 |
