5ERN
Crystal structure of elongation domain of Phomopsis amygdali fusicoccadiene synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 104.867, 104.867, 140.458 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.207 - 2.434 |
R-factor | 0.2252 |
Rwork | 0.223 |
R-free | 0.27590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.653 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.430 |
Number of reflections | 32406 |
<I/σ(I)> | 12.4 |
Completeness [%] | 95.2 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 1.5 M ammonium phosphate, 0.1 M Tris (pH 8.5) |