5ERE
Extracellular ligand binding receptor from Desulfohalobium retbaense DSM5692
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 104.946, 104.946, 140.181 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.187 - 2.000 |
| R-factor | 0.1748 |
| Rwork | 0.172 |
| R-free | 0.22580 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.286 |
| Data reduction software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.103 | |
| Number of reflections | 53557 | |
| <I/σ(I)> | 43.1 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | MCSG-4 #95 (H11): 0.2M calcium acetate, 0.1 M Hepes pH 7.5, 10% PEG8000, 25% glycerol |
