5ERA

Human Connexin-26 (Calcium-free)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	H 3 2
Unit cell lengths	155.727, 155.727, 160.699
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.132 - 3.800
R-factor	0.3037
Rwork	0.300
R-free	0.33340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5er7
RMSD bond length	0.005
RMSD bond angle	0.819
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.360
High resolution limit [Å]	3.300	8.940	3.300
Rmerge	0.082	0.037
Total number of observations	85071
Number of reflections	7398
<I/σ(I)>	5.9
Completeness [%]	99.9	98.9	98.8
Redundancy	7.4	6.8	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M Hepes, 15 mM Sodium formate, 14% (w/v) PEG 3350