5EQX
Crystal structure of human Desmoglein-3 ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 81.909, 44.862, 106.699 |
| Unit cell angles | 90.00, 102.92, 90.00 |
Refinement procedure
| Resolution | 39.918 - 3.050 |
| R-factor | 0.2375 |
| Rwork | 0.236 |
| R-free | 0.27280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.809 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1810) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.260 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Number of reflections | 14791 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 295 | 15% (w/v) PEG10000, 0.15M ammonium formate pH6.7, 0.01M calcium chloride, 30 (v/v) ethylene glycol (cryoprotectant). |
