5EQN
Structure of phosphonate hydroxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.880, 95.090, 138.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.202 - 2.300 |
| R-factor | 0.2197 |
| Rwork | 0.219 |
| R-free | 0.24120 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.940 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.203 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.073 | |
| Number of reflections | 32101 | |
| <I/σ(I)> | 8.03 | |
| Completeness [%] | 99.1 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 289.15 | 0.1 M Bis-Tris pH6.5, 0.2 M NaCl , 25%(w/v) PEG 3350 |
