5EQJ
Crystal structure of the two-subunit tRNA m1A58 methyltransferase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-27 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 138.645, 138.645, 102.727 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.029 - 2.200 |
| R-factor | 0.1847 |
| Rwork | 0.183 |
| R-free | 0.21170 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.129 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 57936 | |
| <I/σ(I)> | 23.5 | 3.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 8.3 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M KCl, 0.1 M Tris HCl pH 8.5, 15% PEG 2000 MME |
