5EQ7
Crystal structure of Medicago truncatula Histidinol-Phosphate Phosphatase (MtHPP) in complex with free phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-26 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.9920 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 86.980, 86.980, 61.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.900 - 1.190 |
| R-factor | 0.111 |
| Rwork | 0.110 |
| R-free | 0.14000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n81 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.220 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.096 | 0.960 |
| Number of reflections | 76240 | |
| <I/σ(I)> | 24.7 | 2 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 34.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | Protein at 19 mg/ml concentration. 15% PEG 3350, 0.2 M diammonium hydrogen phosphate, pH 8.0. Paraton-N as cryoprotectant |
