5EQ6

Pseudomonas aeruginosa PilM bound to AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-15
Detector	BRUKER PHOTON 100
Wavelength(s)	1.5406
Spacegroup name	P 21 21 2
Unit cell lengths	112.600, 151.767, 45.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.500
R-factor	0.2428
Rwork	0.241
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eoy
RMSD bond length	0.005
RMSD bond angle	0.844
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHASER
Refinement software	PHENIX (dev_1639)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.260
Number of reflections	11153
<I/σ(I)>	1.4
Completeness [%]	87.0
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	PEG3350, magnesium chloride, Bis-Tris