5EQ0
Crystal Structure of chromodomain of CBX8 in complex with inhibitor UNC3866
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9786036 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.575, 30.007, 36.104 |
| Unit cell angles | 90.00, 100.17, 90.00 |
Refinement procedure
| Resolution | 35.540 - 1.180 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.18600 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.960 |
| Data scaling software | Aimless (0.5.1) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.540 | 1.200 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Rmerge | 0.038 | 0.343 |
| Number of reflections | 21328 | |
| <I/σ(I)> | 20.5 | 3.7 |
| Completeness [%] | 99.5 | 94.5 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.4 M sodium citrate, 0.1 M HEPES, 5% glycerol |
