5EP4
Structure, Regulation, and Inhibition of the Quorum-Sensing Signal Integrator LuxO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9782 |
Spacegroup name | P 61 |
Unit cell lengths | 73.690, 73.690, 81.792 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.951 - 1.500 |
R-factor | 0.1563 |
Rwork | 0.155 |
R-free | 0.18230 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.665 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.10-2155_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 1.530 |
High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
Rmerge | 0.058 | 0.038 | 0.806 |
Rmeas | 0.062 | 0.041 | 0.866 |
Rpim | 0.022 | 0.014 | 0.315 |
Total number of observations | 311193 | ||
Number of reflections | 40275 | ||
<I/σ(I)> | 7.1 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 7.7 | 7.8 | 7.4 |
CC(1/2) | 0.999 | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M Tris pH 8.0, 0.25 M sodium chloride, 25% w/v PEG3350 |