5EOY

Pseudomonas aeruginosa SeMet-PilM bound to ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97920
Spacegroup name	P 2 21 21
Unit cell lengths	41.575, 110.750, 151.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.073 - 2.500
R-factor	0.2156
Rwork	0.213
R-free	0.26120
RMSD bond length	0.005
RMSD bond angle	0.874
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1639)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.080
High resolution limit [Å]	2.500
Number of reflections	31016
<I/σ(I)>	18.3
Completeness [%]	100.0
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	PEG3350, magnesium chloride, bis-tris