5EOX

Pseudomonas aeruginosa PilM bound to ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-05
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0750
Spacegroup name	P 2 21 21
Unit cell lengths	41.562, 111.355, 150.384
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.710 - 2.400
R-factor	0.2252
Rwork	0.224
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eoy
RMSD bond length	0.005
RMSD bond angle	0.858
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.000
High resolution limit [Å]	2.400
Number of reflections	34804
<I/σ(I)>	30.6
Completeness [%]	100.0
Redundancy	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	PEG3350, magnesium chloride, Bis-Tris