5EOT
Structure of HLA-A2:01 with peptide G13E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.516, 79.358, 57.255 |
Unit cell angles | 90.00, 115.97, 90.00 |
Refinement procedure
Resolution | 39.680 - 2.100 |
R-factor | 0.21078 |
Rwork | 0.209 |
R-free | 0.23678 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mre |
RMSD bond length | 0.008 |
RMSD bond angle | 1.229 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 26570 | |
<I/σ(I)> | 13 | 2.1 |
Completeness [%] | 99.6 | 97.9 |
Redundancy | 3.6 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 30% PEG 5000 MME, 0.1M Tris-HCl pH 8.0, 0.2M lithium sulfate |