5EOS
Crystallizing Strained DNA: Self-Assembly of 3D Crystals Containing a Torsionally-Stressed Component
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1, 1.7 |
| Spacegroup name | H 3 |
| Unit cell lengths | 110.011, 110.011, 83.734 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.500 - 4.700 |
| R-factor | 0.1924 |
| Rwork | 0.190 |
| R-free | 0.21010 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.500 | 4.850 |
| High resolution limit [Å] | 4.700 | 4.750 |
| Rmerge | 0.010 | |
| Number of reflections | 4338 | |
| <I/σ(I)> | 2.6 | 2 |
| Completeness [%] | 95.0 | 90 |
| Redundancy | 1.8 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | Grown by vapor diffusion while treated with a controlled temperature gradient from 333 degs to 277 degs. Ammonium Sulfate, magnesium chloride, Na Cacodylate, magnesium acetate, TRIS |
