5EON

Crystal structure of a de novo antiparallel coiled-coil hexamer - ACC-Hex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.977
Spacegroup name	P 42 21 2
Unit cell lengths	59.256, 59.256, 52.519
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.754 - 1.696
R-factor	0.2003
Rwork	0.198
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eoj
RMSD bond length	0.002
RMSD bond angle	0.389
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.754	1.757
High resolution limit [Å]	1.696	1.696
Rmerge	0.010	0.190
Number of reflections	10805
<I/σ(I)>	31.7	3.42
Completeness [%]	100.0	96
Redundancy	2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	298	0.1 M Na formate, pH 7.3, PEG 3350 29%