Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 100.270, 120.920, 53.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.250 - 1.600 |
| R-factor | 0.14291 |
| Rwork | 0.142 |
| R-free | 0.16707 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iys |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.677 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.250 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.076 | 0.397 |
| Number of reflections | 86406 | |
| <I/σ(I)> | 25 | 6.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 13.6 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 15% PEG 4000, 15% 2-propanol, 0.1M sodium citrate, pH 5.6 |
