5EO9

Crystal Structure of the complex of Dpr6 Domain 1 bound to DIP-alpha Domain 1+2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	120
Detector technology	PIXEL
Collection date	2013-05-18
Detector	PSI PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 21 21 2
Unit cell lengths	130.247, 49.299, 54.707
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.107 - 2.299
R-factor	0.2047
Rwork	0.202
R-free	0.25190
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.683
Data reduction software	HKL-2000 (0.98.708)
Data scaling software	HKL-2000 (0.98.708)
Phasing software	autoSHARP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.299	2.300
Rmerge	0.082	0.684
Number of reflections	16293
<I/σ(I)>	29.4	1.5
Completeness [%]	99.8	96.9
Redundancy	11.2	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	44% PEG400, 0.1 M HEPES pH 7, 0.2 M Li2SO4