5EO2
Structural and biochemical characterization of the hypothetical protein SAV2348 from Staphylococcus aureus in complex with CoA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.980, 127.630, 97.680 |
| Unit cell angles | 90.00, 93.71, 90.00 |
Refinement procedure
| Resolution | 97.480 - 2.500 |
| R-factor | 0.21337 |
| Rwork | 0.212 |
| R-free | 0.24575 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 97.480 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 37843 |
| <I/σ(I)> | 8.73 |
| Completeness [%] | 99.5 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 296 | 0.1M sodium acetate pH4.6, 2M Ammonium sulfate |
